The Z-Competent™ E. coli transformation kit is designed to generate competent E. coli cells for simple and highly efficient E. coli transformation.
- No heat shock required
- Competent cells in <20 mins
- Transformations in <5 mins
- High efficiency transformations
E. coli are grown in SOB medium (supplied with GZ-5), washed and suspended in supplied competent buffer. The bacterial cells are now ready for transformations. The transformation efficiency is 2×10⁸ to 1×10⁹ transformants per μg of pUC19 plasmid. The efficiency of transformation varies depending on E. coli strain and plasmid size. The competent cells can be used immediately or stored for later use.
Informace o dodávce: The kit is supplied with reagents sufficient for processing up to 300×100 µl aliquots of competent cells for transformations (GZ-4) or supplied with reagents and SOB culture media sufficient for processing up to 100×100 µl aliquots of competent cells (GZ-5).