Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.
- Provides a simple and versatile method for protein extraction from mammalian cells
- Compatible with electrophoresis and chromatographic applications
- Compatible with most enzyme assays including: Reporter gene assays (ß-galactosidase, luciferase, chloramphenicol acetyltransferase); Kinases (protein kinase C, protein kinase A, tyrosine kinase); Immunoassays (ELISA, Western blots, RIA)
- Suitable for most applications including enzyme and protein purification applications, electrophoresis, Western blotting and 2D-gel analysis