FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit for the fractionation of nuclear and cytoplasmic proteins from cells and tissues.
- Includes chaotropic extraction buffer for solubilisation of nuclear and cytoplasmic proteins
- Fully compatible with 2D analysis
The FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit is supplied with a strong chaotropic extraction buffer to solubilise both cytoplasmic and nuclear proteins, which is fully compatible with 2D gel electrophoresis.
The FOCUS™ Cytoplasmic and Nuclear Protein Fractionation kit is based on the Nuclear and Cytoplasmic Extraction Kit; however, it has been modified to be fully compatible with 2D electrophoresis and subsequent downstream processes.
The kit is provided with reagents necessary for fractionation of cytoplasmic and nuclear proteins as well as solubilisation buffer suitable for IEF/2D gels for better spot resolution. The kit is designed for >50 preps, depending on the tissue used, where one prep is 20×10⁶ mammalian cells or 100 mg mammalian tissue.