IMAC Sepharose™ High Performance media is easily charged with the metal ion of choice (e.g. Cu²⁺, Zn²⁺, Ni²⁺ or Co²⁺ for immobilised metal ion affinity chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.
- For optimising purification of histidine-tagged proteins when Ni²⁺ is not the best choice of metal ion
- Small bead size (34 μm) for high performance purification
- High binding capacity
These metal ions will bind to the covalently immobilised chelating ligand on the Sepharose™.