HiTrap™ Con A 4B is a ready-to-use column prepacked with Con A Sepharose™ 4B, a medium for convenient separation and purification of glycoproteins, polysaccharides, and glycolipids.
- Con A Sepharose™ is concanavalin A coupled to Sepharose™ 4B
- Can be operated with a syringe, a peristaltic pump, or a liquid chromatography system such as ÄKTA design
Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl, and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A.
HiTrap™ Con A 4B columns can be operated with a syringe, a peristaltic pump, or a liquid chromatography system such as ÄKTA design.