Immunoprecipitation is a highly specific and effective technique for analytical separations of target antigens from crude cell lysates. In combination with other techniques such as SDS-PAGE and immunoblotting immunoprecipitation can detect and quantify antigens, determine relative molecular weights, monitor protein turnover and posttranslational modifications, and check for enzyme activity.
- High binding capacity with minimal nonspecific adsorption
- Low leakage
The proteins are immobilized on Sepharose™ 4 Fast Flow by the cyanogen bromide method. Protein A and protein G have different binding selectivities depending on the origin of the IgG.