Tisk…

Genomic DNA extraction, illustra™ Nucleon™ HT

Dodavatel: Cytiva
RPN8509EA 8500 CZK
GEHERPN8509
Genomic DNA extraction, illustra™ Nucleon™ HT
The illustra™ Nucleon™ HT kit facilitates the simple, phenol-free extraction of genomic DNA from paraffin-embedded sections and hard tissue requiring proteinase K digestion.

  • Each kit is designed to recover high quality DNA from 50 preparations of up to 25 mg of hard tissue
  • Consistent chloroform extraction of high-quality, amplifiable DNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections
  • User friendly protocol enables efficient processing of large numbers of samples

As DNA purified from FFPE tissue slides is highly degraded, damaged, and available only in small quantities, little molecular analysis has been performed on such samples. illustra™ Nucleon™ HT protocols have been designed to prepare high-quality DNA from hard tissues and FFPE tissue sections that is suitable for genomic DNA amplification. Together, these tools enable molecular analysis of genome integrity and DNA copy number changes from FFPE tissue slides.

The Nucleon™ proprietary resin is added following cell lysis, deproteinization with sodium perchlorate, and a single chloroform extraction. Aspiration of the top layer of nucleic acids is simplified and volume of usable sample maximised by a barrier formed between the two layers. The recovered DNA is suitable for a variety of molecular biology applications, including whole genome amplification.

Informace pro objednávání: Each kit is designed to recover high quality DNA from 50 preparations of up to 25 mg of hard tissue.
Objednat

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Více About VWR