Chromolith® DIOL columns are useful as an intermediate polarity media between C18 and silica. Diol functionalised media is less polar and can have different selectivity. Chromolith® DIOL can be used with a wide range of solvents in both normal and reverse phases. In normal phase mode, the diol phase provides an alternative selectivity compared to silica whilst additionally demonstrating longer retention times that help to obtain separation. In reverse phase mode, compounds such as water-soluble vitamins, sugars or amino acids are easily baseline separated using simple mobile phases. Chromolith® DIOL columns have long lifetimes within pH range of 2,5 to 7,5, high matrix tolerance and speed of analysis.