Buffer, HEPES solution (1 mol/L), Corning®

Dodavatel: Corning
25-060-CIEA 11340 CZK
Buffer, HEPES solution (1 mol/L), Corning®
Pufry Pufry pro tkáňové kultury
HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

Unlike the low pKa of bicarbonate, HEPES has a pKa of 7.66 making it a much stronger buffer in the pH 7.2-7.6 range than bicarbonate buffer. Potential disadvantages of HEPES include toxicity over 100 mM and lack of nutritive value to the cell.

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