Luminescent enzyme substratesDodavatel: Biotium
10127.EA 14710 CZK10127. 10126. 10126-50UG 10127-50UG
Luminescent enzyme substrates
Systémy pro spojování a značení proteinů
D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidised by an ATP-dependent process, catalysed by luciferase.
Luciferase is encoded by the luc gene, widely used as a reporter gene in cells.
In addition, luciferin/luciferase has been used to measure 10 - 15 molar quantity of ATP. D-luciferin, potassium salt is a water-soluble form of D-luciferin.
Because of the intrinsic low background of chemiluminescence technique, detection of the luc gene expression can be made at very low level.
Coelenterazines are poorly water soluble, which complicates the formulation of these substances for use in live animals. Aquaphile™ coelenterazines are bioluminescence substrates in a special formulation format that are readily dissolved in water or PBS buffer for in vivo use. Coelenterazine is a substrate for Renilla (sea pansy) luciferase, as well as other enzymes such as Gaussia, Metridia, and Oplophorus luciferases. Renilla luciferase catalyses coelenterazine oxidation by oxygen to produce light, and is a widely used reporter gene for luminescence based assays.
Coelenterazine native is the natural substrate for Renilla luciferase. In addition, over a dozen of coelenterazine analogues have been synthesised that can function as substrates for Renilla luciferase, and have different luminescent properties. In addition to quantum yields, emission wavelength can be an important factor when luciferase is used in combination with a fluorescent protein such as GFP for bioluminescent resonance energy transfer (BRET), an important application for the studies of protein-protein interactions.