Tisk…

BCIP/NBT alkaline phosphatase substrate

Dodavatel: Rockland Immunochemicals

ROCKNBT-100EA 6010 CZK
ROCKNBT-100
BCIP/NBT alkaline phosphatase substrate
BCIP/NBT

BCIP/NBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.


BCIP/NBT Alkaline Phosphatase Membrane Substrate is a useful detection reagent for immunoblotting. The substrate is ready to use. To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquoise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.


Storage Condition: Store container at 4° C prior to opening. Protect BCIP/NBT Membrane Alkaline Phosphatase Substrate from moisture and light.

Objednat

Výsledky specifikačního testu

pH 9.28 +/- 0.1
QC Raw Material PASS
Absorbance check of final product PASS
Performance Data per Immunoblot PASS

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Více About VWR