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Anti-GAPDH Rabbit Polyclonal Antibody

Dodavatel: Thermo Scientific
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Suggested positive control: antigen standard for GAPDH (transient overexpression lysate). GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amounts and which is required at all times for an important house keeping functions, levels of GAPDH mRNA are often used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH as loading controls for western blotting experiments. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also be reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.

Type: Primary
Antigen: GAPDH
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Primates, Rat, Mouse, Human



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100 µL 100 µl PIEAPA116777 Blocked
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Produktová specifikace

Symbol antigenu GAPDH
Název antigenu Glyceraldehyde-3-phosphate dehydrogenase
Reaktivita Primates, Rat, Mouse, Human
Spojení Unconjugated
Klonovalita Polyclonal
Druh protilátky Primary
Hostitel Rabbit
Isotyp IgG
Western Blot Yes
Imunochemie Yes
Imunofluorescence Yes
Průtoková cytometrie Yes
Synonyma antigenu G3PD, G3PDH, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase
Gene ID 14433, 24383, 2597
Koncentrace 0.2 mg/ml
Imunogen Residues between 150 and 200 of human Glyceraldehyde-3-Phosphate Dehydrogenase (GeneID 2597)
Čištění Antigen affinity chromatography
Forma Liquid
Skladovací pufr TBS, 0.1% BSA, 0.09% sodium azide
Skladovací teplota 4° C, do not freeze
Transportní teplota Ice
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