The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. p53 can apparently be phosphorylated by ATM, ATR, and DNA-PK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage.
Recommended Dilutions: Western Blot: 0.5-4 µg/ml; Immunoprecipitation: 5-10 µg/ml; and Immunohistochemistry: 20 µg/ml
Reactivity: Human, Mouse, Rat