This gene encodes a nuclear protein which is involved in cell cycle regulation. The encoded protein is a ubiquitin ligase capable of ubiquinating PCNP (PESTcontaining nuclear protein), and together they may play a role in tumorigenesis. The encoded protein contains an NIRF_N domain, a PHD finger, a set and ring associated (SRA) domain, and a RING finger domain and several of these domains have been shown to be essential for the regulation of cell proliferation. This protein may also have a role in intranuclear degradation of polyglutamine aggregates. Alternative splicing results in multiple transcript variants, some of which are nonprotein coding. [Provided by RefSeq, Feb 2012].
Recommended Dilutions: Western Blot: 1:500-1:2000, IHC: 1:50-1:200, Immunofluorescence: 1:20-1:50.