The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 136 amino acid chains, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and murine cells.
Recommended Dilutions: 1) Western Blot: Use 0.1-0.2 µg/ml. The detection limit for recombinant human IL-17A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.<br>2) ELISA: Use 0.5 - 2.0 ?g/ml (100 ?l/well antibody solution) <br>3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17A (50.0 ng/ml; a concentration of 0.9-1.3 ?g/ml of this antibody is required.<br>4) Immunohistochemistry: 0.25 µg/ml with an overnight incubation at 4°C.