Human IL-33 is a proinflammatory protein that shares structural and functional characteristics with the IL-1 cytokine family. It binds and signals through the IL-1RL1/ST2 receptor activating NF-kappaB and MAP kinases. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities.
Recommended Dilutions: 1) Western Blot: Use 0.1-0.2 µg/ml. The detection limit for recombinant human IL-33 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.<br>2) ELISA: Use 0.5 - 2.0 ?g/ml (100 ?l/well antibody solution) <br>3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-33 (1.0 ng/ml; a concentration of 0.2-0.4 ?g/ml of this antibody is required.<br>4) Immunohistochemistry: 0.125 ?g/ml-0.25 ?g/ml with an overnight incubation at 4°C