The ImmunoReagents' Allophycocyanin (APC) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields. It is excited by laser lines at 594 and 633 nm, with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm. APC is a large molecule that is chemically crosslinked to use in fluorescence-based detection, primarily for flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability.
This conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(?): · 1:20 - 1:2,000 for most applications
Antigen: IgG H&L, F(ab)'2 Fragment
Conjugation: APC (Allophycocyanin)