Pierce™ Nickel coated plates are ideal for analysing polyhistidine-tagged fusion proteins by ELISA based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.
- Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
- Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
Detection limit: 1 ng of polyhistidine fusion protein
Binding capacity: ~9 pmol His-tagged protein (27 kDa) per well