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NHS-Azide and NHS-Phosphine Reagents, Pierce™

Dodavatel: Thermo Scientific
Pierce
PIER88900EA 13490 CZK
PIER88900 PIER88902 PIER26130
NHS-Azide and NHS-Phosphine Reagents, Pierce™
Systémy pro spojování a značení proteinů
The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.
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