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Ubiquitin enrichment kit, Pierce™

Dodavatel: Thermo Fisher Scientific
PIER89899EA 21020 CZK
PIER89899
Ubiquitin enrichment kit, Pierce™
Systémy pro extrakci proteinů Protein Extraction Kits
The Pierce™ Ubiquitin Enrichment Kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.

  • Takes less than 45 minutes hands-on time for binding and recovery of ubiquitinated proteins
  • Sample incubation from 2 hours to overnight allows assay to be completed in several hours or in less than 30 minutes after overnight incubation
  • Compatible with all cell lysis products and standard RIPA formulations
  • Easily processes 1 to 15 samples concurrently

The fractions recovered from this Ubiquitin Enrichment Kit can be subsequently analysed to determine the amount of general ubiquitin conjugates present or to identify a specific protein of interest by Western blotting. The assay protocol is fast and straightforward, allowing for isolation of polyubiquitinated proteins and the fractionation of monoubiquitinated species in the resin flow-through. It provides exceptional performance, as demonstrated by enriching an epoxomicin-treated HeLa lysate for poly ubiquitinated proteins.

Informace o dodávce: The kit contains polyubiquitin positive control (1000X, 50 µl), anti-ubiquitin antibody (rabbit antiserum, 5 µl), polyubiquitin affinity resin slurry (300 µl), TBS pack (makes 500 ml), and spin columns (18) and accessories.
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