The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.
- Optimised labeling reactions switch S-nitrosocysteine post translational modifications with iodoTMT™ reagent for easy detection by Western blot using anti-TMT™ antibody
- Uses a non-biological iodoTMT™ reagent for labeling instead of HPDP-biotin, resulting in less background during Western blot detection
- Labeling method is compatible with alternative methods of analysis, including enrichment via anti-TMT™ resin and multiplex quantitation by mass spectrometry
- All needed reagents included one kit
The Pierce™ S-Nitrosylation Western Blot Kit contains a cell lysis/reaction buffer, a sulfhydryl-reactive blocking agent, a reducing agent, a labeling agent and a detection monoclonal antibody. Unmodified cysteines are first blocked using a sulfhydryl-reactive compound (MMTS). S-nitrosylated cysteines are then selectively reduced with ascorbate in HENS Buffer for specific labeling with iodoTMTzero™ reagents, which irreversibly bind to the cysteine thiol that was S-nitrosylated. Detection of the TMT™ reagent-modified proteins is facilitated using an anti-TMT™ antibody.
Informace o dodávce: The kit includes HENS Buffer (100 ml), methyl methanethiosulfonat (MMTS, 200 mg), sodium ascorbate (20 mg), iodoTMTzero™ label reagent (2×0,2 mg) and anti-TMT™ antibody (100 µl).
