Substrate for western blotting, 1-Step™ NBT/BCIP plus suppressor

Dodavatel: Thermo Scientific
PIER34070EA 4170 CZK
Substrate for western blotting, 1-Step™ NBT/BCIP plus suppressor
Činidla pro elektroforézu Blotting - činidla
The 1-Step NBT/BCIP plus suppressor is a one-component alkaline phosphatase substrate that inhibits endogenous phosphatase activity. This substrate reacts with calf intestinal alkaline phosphatase to produce a dark purple precipitate and is ideal for immunohistochemistry and in situ hybridisation applications.

Two forms of alkaline phosphatase exist in mammals: One form is distributed in a variety of tissues such as the kidney, liver, placenta, bone and tumour; the other form resides in the intestine. These two forms are affected differently by inhibitors. Levamisole added to the substrate inhibits endogenous non-intestinal alkaline phosphatases but does not affect the intestinal form commonly used as a detection label.

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