Amino acid modification reagents, sulfhydryl addition kit, Pierce™

Dodavatel: Thermo Scientific
PIER23460EA 10610 CZK
Amino acid modification reagents, sulfhydryl addition kit, Pierce™
Systémy pro purifikaci proteinů Protein Purification Kits
The Pierce™ Sulfhydryl Addition Kit contains all necessary components to introduce free sulfhydryl groups into any primary amine-containing molecule, purify the modified molecule and quantify the added sulfhydryl groups.

The Sulfhydryl Addition Kit uses SATA (N-succinimidyl S-acetylthioacetate) as a means of adding sulfhydryls to molecules. SATA contains an N-hydroxysuccinimide (NHS) ester that forms a stable amide bond with primary amines. Once the covalent linkage is formed, the modified molecule can be stored and the sulfhydryl group de-protected when needed. The reaction conditions for SATA are mild and non-denaturing, which preserves protein activity.

Informace o dodávce: The kit includes SATA (2 mg); hydroxylamine-HCl (5 mg); 10X conjugation buffer stock (20 ml); phosphate buffered saline pack (1 pack); dimethylformamide (DMF, 1ml); dextran desalting column (1×5 ml); column extender (1); Ellman’s reagent (DTNB, 2 mg); and cysteine-HCl H2O (20 mg).

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