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Reactive quencher dyes

Dodavatel: Biotium

BTIU80502EA 9150 CZK
BTIU80502 91051. 80501. 80500.
Reactive quencher dyes
Systémy pro spojování a značení proteinů
Reactive quenchers that act as acceptors in fluorescence resonance energy transfer (FRET) applications.

  • Thiol- and amine-reactive quenchers
  • One Sample Fluorescence Resonance Energy Transfer (OS-FRET) and FRET quenchers

NFQ1, a non-fluorescent quencher, is used for the One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method employing a non-fluorescent methanethiosulfonate-linked acceptor that can be reversibly coupled to a target sulfhydryl residue via a disulfide bond. After the quenched donor emission is quantitated the acceptor is removed by reduction, enabling measurement of unquenched donor emission in the same sample.

The thiol-reactive quencher, CFQ520 maleimide, has a broad and intense absorption (approximately 520 nm) but no fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.

The amine-reactive quencher, CFQ520 succinimidyl ester, has a broad and intense absorption (approximately 520 nm) but no fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.

N-(6-Aminohexyl)maleimide is a cross-linking reagent that can react with an amine-reactive group and a thiol group.
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