Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transformation and has been successfully used to transform linear DNA into Pichia pastoris for protein expression studies.
- Easy to use – can be completed in less than 90 minutes
- High transformation efficiency routinely greater than 10⁴ transformants per µg of DNA
- Scalability allows high efficiency transformation needed in two hybrid screens
The kit provides all of the reagents required to perform an easy and quick non electrical method for transforming yeast with linear or plasmid DNA. Host cells are made competent by treatment with a lithium/caesium acetate mixture.
Informace o dodávce: Kit includes pH 7,5 TE; lithium/acesium acetate; carrier DNA, 10 mg/ml; sterile histamine solution; TE/cation MIXX; and sterile solution SOS.