NucSpot® 470 1000X in DMSO nuclear stainDodavatel: Biotium
NucSpot 470 is cell membrane-impermeant nuclear-specific green fluorescent DNA stain designed for the FITC channel. It is virtually non-fluorescent in the absence of DNA, but fluoresces bright green upon DNA binding.
- Excitation/Emission 460/546 nm (with DNA)
NucSpot 470 specifically stains the nucleus of fixed and permeabilised cells while other green nucleic acid stains like TOTO®, TO-PRO®, or SYTOX® dyes stain both the nucleus and cytoplasm.
It also can be used to selectively stain dead cells in living cultures.
NucSpot 470 has green fluorescence that can be imaged using standard settings for FITC. With excitation at 460 nm, it also is an excellent match for instruments with blue LED excitation sources.