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Triglyceride quantification kit, colorimetric and fluorometric

Dodavatel: BioVision
BIOVK622-100EA 16690 CZK
BIOVK622-100
Triglyceride quantification kit, colorimetric and fluorometric
Triglycerides (TG) are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source.

  • Measurement of triglycerides in various tissues, cells and other biological fluids
  • Mechanistic study of cardiovascular diseases
  • Analysis of lipid metabolism

TG are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of TG are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis. The triglyceride quantification kit provides a sensitive, easy assay to measure TG concentration in a variety of samples.

In the assay, TG are converted to free fatty acids and glycerol. The glycerol is then oxidised to generate a product which reacts with the probe to generate colour (spectrophotometry at λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect two pmol to ten nmol (or 2 to 10000 µM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.

Informace o dodávce: Kit contains triglyceride assay buffer 25 ml; triglyceride probe (in DMSO, anhydrous) 200 µl; lipase 1 vial; triglyceride enzyme mix (lyophilised) 1 vial and triglyceride standard (1 mM) 0,3 ml.
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