Rat tail collagen coating solution enhances cell attachment and proliferation, or promotion of cell-specific morphology and function. Commonly used for endothelial cells, hepatocytes, muscle cells, and a variety of other cell types. This collagen solution is prepared in 0,02 M acetic acid at 50 ug/ml and sterile filtered.
Coat tissue cultureware with rat tail collagen solution to culture surface ratio of 5 ug/cm². For example: 125 μg (2,5 ml) for a T-25 flask; 10,5 μg (210 μl) for each well of a 24-well plate.
Coated tissue cultureware may be used immediately or air-dried and stored at 4 °C for up to one week.