Aconitase (aconitate hydratase; EC 4,2,1,3) is an iron-sulphur protein containing an [Fe₄S₄]² cluster that catalyses the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process.
- Simple procedure which takes less than 40 minutes
- Fast and convenient
- Species reactivity: Mammalian
Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage.
Aconitase assay kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colourless probe into an intensely coloured form with a λmax at 450 nm.
Informace o dodávce: Kit contains assay buffer, substrate, developer, enzyme mix, cysteine-HCl, (NH₄)₂Fe(SO₄)₂ and isocitrate standard.
Upozornení: Research purpose only, not for human use.