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Plant protein extraction reagents, P-PER

Dodavatel: Thermo Scientific
PIER89803EA 10700 CZK
PIER89803
Plant protein extraction reagents, P-PER
The P-PER Plant Protein Extraction Kit effectively extracts protein from all kinds of dry and fresh plant tissue without liquid nitrogen. The P-PER Kit includes an organic lysing reagent and two aqueous reagents that effectively lyse plant cells and solubilise protein without harsh mechanical lysis aids, such as a mortar and pestle. Plant protein extracts are prepared in just 10 minutes, and protein yields exceed conventional extraction methods (e.g., freeze/grinding in liquid nitrogen) and other commercially available plant protein extraction reagents. The resulting protein extracts are compatible with a variety of downstream applications including 1D and 2D gel electrophoresis, immunoprecipitation, Western blotting, activity assays and protein purification.

  • Versatile - works with multiple plant organs (leaf, stem, root, seed and flowers); multiple plant species (Arabidopsis, tobacco, maize, soybeans, peas, spinach and other plant tissues); and fresh, frozen and dehydrated plant tissues
  • Convenient - requires no liquid nitrogen/freeze-grinding, Dounce homogenization, blade-shearing or glass-bead agitation for cell disruption; however, the P-PER Kit is compatible with these alternative mechanical aids
  • Fast and ready-to-use - perform plant cell lysis and protein extraction in 10 minutes; protein extract does not require filtration through cheesecloth
  • Recovers active protein - assays show extracted proteins are functional

Informace o dodávce: Kit contains P-PER Reagent A, 20 ml; P-PER Reagent B, 225 µl; P-PER Reagent C, 20 ml; PP Mesh Bags, 20 each.
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